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オゾンとLPS誘発マウス急性肺損傷時の肺細胞適応の可視化
JoVE Journal
Immunology and Infection
Author Produced
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JoVE Journal Immunology and Infection
Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury
DOI:

14:48 min

March 21, 2021

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Chapters

  • 00:00Introduction
  • 01:33Protocol: Chapter I. Ozone and LPS Exposures for Induction of Murine ALI
  • 02:33Protocol: Chapter II. Sample Collection
  • 08:55Protocol: Chapter III. Sample Processing
  • 10:17Representative Results
  • 14:24Conclusion

Summary

Automatic Translation

オゾンと細菌性内毒素を組み合わせたマウスは、好中球を含む広域細胞死を示す。細胞骨格ラメリポディアの破壊、気管支-肺胞洗浄における複雑なV ATPシンターゼサブユニットβおよびアンジオスタチンの細胞発現の増加、肺免疫応答の抑制および好中球の遅れなど細胞適応を観察した。

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