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Visualisera lungcellsanpassningar under kombinerad ozon- och LPS-inducerad murin akut lungskada
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury
DOI:

14:48 min

March 21, 2021

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Chapters

  • 00:00Introduction
  • 01:33Protocol: Chapter I. Ozone and LPS Exposures for Induction of Murine ALI
  • 02:33Protocol: Chapter II. Sample Collection
  • 08:55Protocol: Chapter III. Sample Processing
  • 10:17Representative Results
  • 14:24Conclusion

Summary

Automatic Translation

Kombinerade ozon- och bakteriella endotoxinexponerade möss uppbar bred celldöd, inklusive neutrofiler. Vi observerade cellulära anpassningar såsom störningar av cytoskeletal lamellipodia, ökat cellulära uttryck av komplexa V ATP syntas underavdelning β och angiostatin i broncho-alveolar lavage, undertryckande av lung immun svar och fördröjd neutrophil rekrytering.

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