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用于评估啮齿动物和人类脑组织中行为素聚合状态的基于时间的荧光光谱分析
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JoVE Journal Neuroscience
A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues
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用于评估啮齿动物和人类脑组织中行为素聚合状态的基于时间的荧光光谱分析

DOI:
10.3791/62268-v

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06:54 min

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June 03, 2021

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,
1Department of Anatomy, School of Biomedical Sciences,University of Otago

Chapters

  • 00:00Introduction
  • 01:49Preparation of Buffers and Reagents
  • 02:02Preparation of Samples
  • 02:56KCl-mediated Depolarization of Isolated Synaptic Terminals
  • 03:21Fixation and Phalloidin Staining of Samples
  • 04:40Fluorometric Analysis and Light Scattering
  • 05:20Data Analysis
  • 06:19Conclusion

Summary

Automatic Translation

Automatic Translation

我们报告了一种简单、省时和高通量荧光光谱分析，用于从啮齿动物和人类受试者脑组织 中对 活体细丝进行定量。

Tags

ActinCytoskeletonF-actinG-actinPolymerizationFluorescence SpectroscopyPhalloidinSynaptic TerminalsSynaptosomesSynaptoneurosomesNeuropathologyNeuronal Physiology

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