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Ein zeiteffizienter Fluoreszenzspektroskopie-basierter Assay zur Bewertung des Actin-Polymerisationsstatus in Nagetier- und menschlichem Hirngewebe
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Chapters
- 00:00Introduction
- 01:49Preparation of Buffers and Reagents
- 02:02Preparation of Samples
- 02:56KCl-mediated Depolarization of Isolated Synaptic Terminals
- 03:21Fixation and Phalloidin Staining of Samples
- 04:40Fluorometric Analysis and Light Scattering
- 05:20Data Analysis
- 06:19Conclusion
