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En tidseffektiv fluorescensspektroskopibaseret analyse til evaluering af Actin polymeriseringsstatus i gnavere og humane hjernevæv
JoVE Journal
Neuroscience
Author Produced
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JoVE Journal Neuroscience
A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues
DOI:

06:54 min

June 03, 2021

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Chapters

  • 00:00Introduction
  • 01:49Preparation of Buffers and Reagents
  • 02:02Preparation of Samples
  • 02:56KCl-mediated Depolarization of Isolated Synaptic Terminals
  • 03:21Fixation and Phalloidin Staining of Samples
  • 04:40Fluorometric Analysis and Light Scattering
  • 05:20Data Analysis
  • 06:19Conclusion

Summary

Automatic Translation

Vi rapporterer om en enkel, tidseffektiv og højgennemstrømningsspektroskopibaseret analyse til kvantificering af aktinfilamenter i ex vivo biologiske prøver fra hjernevæv fra gnavere og mennesker.

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