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Journal
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Immunology and Infection
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流式细胞术分析人髓系U937细胞中SAMHD1限制性
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells
Please note that all translations are automatically generated.
Click here for the English version.
流式细胞术分析人髓系U937细胞中SAMHD1限制性
DOI:
10.3791/62502-v
•
09:43 min
•
June 13, 2021
•
Paula Ordonez
,
Kate N. Bishop
,
Jonathan P. Stoye
,
Harriet Cordelia Theed Groom
1
Retrovirus-Host Interactions Laboratory
,
The Francis Crick Institute
,
2
Retroviral Replication Laboratory
,
The Francis Crick Institute
,
3
Sidney Sussex College, Department of Medicine
,
University of Cambridge
Chapters
00:05
Introduction
00:35
Flow Cytometry Analysis
04:18
Data Analysis
07:50
Results: Analysis of the Restriction Ratio in the Optimal and Suboptimal Data
09:15
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里描述的是一种确定HIV-1限制程度的既定方法，由细胞抑制蛋白SAMHD1。人髓系U937细胞用共表达YFP的SAMHD1表达载体转导，分化，然后用HIV-RFP攻击。限制水平由流式细胞术分析确定。
Tags
SAMHD1
Flow Cytometry
U937 Cells
HIV Infection
Macrophages
Cellular Factors
Multicolor Flow Cytometry
Cytometer
Flow Rate
Forward Scatter
Side Scatter
YFP
RFP
Compensation Controls
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