/
/
2D og 3D humant inducerede pluripotente stamcellebaserede modeller til at dissekere primær ciliuminddragelse under neokortikal udvikling
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Imagine Institute, INSERM UMR 1163,Université de Paris, 2Imagine Institute, iPSC Core Facility, INSERM UMR U1163,Université de Paris, 3Necker Bio-image Analysis platform of the SFR Necker, 4Imagine Institute, Cell Imaging Platform, INSERM-US24-CNRS UMS 3633 Structure Fédérative de Recherche Necker, INSERM UMR U1163,Université de Paris, 5Fédération de Génétique, Hôpital Necker-Enfants Malades,Assistance Publique Hôpitaux de Paris, 6Pediatric Neurology,APHP- Necker Enfants Malades Hospital
Chapters
- 00:07Introduction
- 01:42Generation of 2D hIPS Cell-Based Models of Neocortical Development
- 02:56Generation of Dorsal Forebrain Organoids
- 04:56In toto Immunolabeling, Clearing, and Lightsheet Acquisition of Dorsal Forebrain Organoids
- 07:00Immunostaining and Clearing of Free-Floating Sections of Dorsal Forebrain Organoids
- 09:20Representative Results
- 13:11Conclusion
Vi præsenterer detaljerede protokoller til generering og karakterisering af 2D og 3D humant induceret pluripotent stamcelle (hIPSC) -baserede modeller for neokortikal udvikling samt komplementære metoder, der muliggør kvalitativ og kvantitativ analyse af primær cilium (PC) biogenese og funktion.
