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Isolering af livmoder medfødte lymfoidceller til analyse af flowcytometri
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry

Isolering af livmoder medfødte lymfoidceller til analyse af flowcytometri

DOI:
10.3791/62670-v

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09:02 min

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October 14, 2021

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, , , , ,
1Department of Obstetrics and Gynaecology, National Institute for Health Research Cambridge Biomedical Research Centre,University of Cambridge School of Clinical Medicine, 2Centre for Trophoblast Research,University of Cambridge

Chapters

  • 00:04Introduction
  • 00:55Mechanical and Enzymatic Digestion of the Uterus
  • 02:35Processing of the Uterus into a Single Cell Suspension
  • 05:22Red Blood Cell Lysis
  • 06:13Results: FACS Analysis of Innate Lymphoid Cells (ILCs)
  • 08:06Conclusion

Summary

Automatic Translation

Automatic Translation

Dette er en metode til at isolere livmoder lymfoide celler fra både gravide og ikke-gravide mus. Denne metode kan bruges til flere downstream applikationer såsom FACS phenotyping, celle sortering, funktionelle assays, RNA-seq, og proteomics. Protokollen her viser, hvordan man fænotype gruppe 1 livmoder medfødte lymfoidceller ved flow cytometri.

Tags

Uterine Innate Lymphoid CellsFlow CytometryPregnantNon-pregnantLymphocyte IsolationCell ViabilityFetus RemovalEnzymatic DigestionEDTA PBSCell Strainer

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