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测量重组透气溶性细胞中线粒体底物通量 O-透化细胞
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Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells
Please note that all translations are automatically generated.
Click here for the English version.
测量重组透气溶性细胞中线粒体底物通量 O-透化细胞
DOI:
10.3791/62902-v
•
06:17 min
•
August 13, 2021
•
Moustafa Elkalaf
2
,
Karolína Vaněčková
2
,
Pavla Staňková
,
Zuzana Červinková
,
Jan Polák*
2,3
,
Otto Kučera*
1
1
Department of Physiology, Faculty of Medicine in Hradec Králové
,
Charles University
,
2
Department of Pathophysiology, Third Faculty of Medicine
,
Charles University
,
3
Department of Internal Medicine
,
University Hospital Kralovske Vinohrady
Chapters
00:04
Introduction
01:03
Day 1
03:00
Day 2
04:57
Results: Mitochondrial Respiration Measurement
05:33
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这项工作中，我们描述了一种改进的方案，以使用重组产气溶酶O与基于微孔板的呼吸测定法一起测试线粒体呼吸底物通量。通过该协议，我们展示了二甲双胍如何影响两种不同肿瘤细胞系的线粒体呼吸。
Tags
Mitochondrial Substrate Flux
Recombinant Perfringolysin O-permeabilized Cells
Cancer Cell Response
Metformin Treatment
Mitochondrial Metabolism
Seahorse XF 96 Cell Culture Microplate
Oligomycin
FCCP
Rotenone Antimycin A
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