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由去细胞化肺切片制备的工程肺组织
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Engineered Lung Tissues Prepared from Decellularized Lung Slices
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由去细胞化肺切片制备的工程肺组织

DOI:
10.3791/63151-v

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08:01 min

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January 21, 2022

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, , ,
1Department of Biomedical Engineering,Yale University, 2Yale School of Medicine, 3Department of Cellular and Molecular Physiology,Yale School of Medicine, 4Department of Anesthesiology,Yale School of Medicine

Chapters

  • 00:04Introduction
  • 00:35Harvest of Rat Lungs
  • 01:15Cutting and Clipping the Lung Slices
  • 02:25Slice Decellularization
  • 03:35Slice Recellularization and Culture
  • 06:20Results: Visualization of the Structure of Decellularized Lung Scaffolds and Recellularized Engineered Lung Tissues (ELTs)
  • 07:35Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了一种通过用肺泡上皮2型细胞，成纤维细胞和内皮细胞重新填充去细胞化精密切割的肺切片来产生可重复的小规模工程肺组织的方法。

Tags

Extracellular MatrixLung SliceDecellularizationCassetteAgaroseHBSSPBSRazor BladeOrbital Shaker

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