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Tejidos pulmonares diseñados preparados a partir de rodajas pulmonares descelularizadas
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Engineered Lung Tissues Prepared from Decellularized Lung Slices
DOI:

08:01 min

January 21, 2022

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Chapters

  • 00:04Introduction
  • 00:35Harvest of Rat Lungs
  • 01:15Cutting and Clipping the Lung Slices
  • 02:25Slice Decellularization
  • 03:35Slice Recellularization and Culture
  • 06:20Results: Visualization of the Structure of Decellularized Lung Scaffolds and Recellularized Engineered Lung Tissues (ELTs)
  • 07:35Conclusion

Summary

Automatic Translation

Este protocolo describe un método para generar tejidos pulmonares reproducibles y diseñados a pequeña escala, mediante la repoblación de rodajas pulmonares descelularizadas cortadas con precisión con células epiteliales alveolares tipo 2, fibroblastos y células endoteliales.

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