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Journal
/
Bioengineering
/
脱細胞化肺スライスから調製された人工肺組織
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Engineered Lung Tissues Prepared from Decellularized Lung Slices
Please note that all translations are automatically generated.
Click here for the English version.
脱細胞化肺スライスから調製された人工肺組織
DOI:
10.3791/63151-v
•
08:01 min
•
January 21, 2022
•
Katherine L. Leiby
2
,
Ronald Ng
,
Stuart G. Campbell
3
,
Laura E. Niklason
4
1
Department of Biomedical Engineering
,
Yale University
,
2
Yale School of Medicine
,
3
Department of Cellular and Molecular Physiology
,
Yale School of Medicine
,
4
Department of Anesthesiology
,
Yale School of Medicine
Chapters
00:04
Introduction
00:35
Harvest of Rat Lungs
01:15
Cutting and Clipping the Lung Slices
02:25
Slice Decellularization
03:35
Slice Recellularization and Culture
06:20
Results: Visualization of the Structure of Decellularized Lung Scaffolds and Recellularized Engineered Lung Tissues (ELTs)
07:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
このプロトコールは、脱細胞化された精密切断肺スライスに肺胞上皮2型細胞、線維芽細胞、および内皮細胞を再移入することによって、再現性のある小規模の操作された肺組織を生成する方法を記載している。
Tags
Extracellular Matrix
Lung Slice
Decellularization
Cassette
Agarose
HBSS
PBS
Razor Blade
Orbital Shaker
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