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JoVE Journal Developmental Biology
Microdissection and Dissociation of the Murine Oviduct
 

Microdissection and Dissociation of the Murine Oviduct: Individual Segment Identification and Single Cell Isolation

Article DOI: 10.3791/63168-v • 07:44 min • November 4th, 2021
November 4th, 2021 •
1, 1, 2, 1
1Division of Biomedical Sciences, School of Medicine, University of California, Riverside, 2Microscopy and Imaging Core Facility, University of California, Riverside

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Summary
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A method for microdissection of the mouse oviduct that allows collection of the individual segments while maintaining RNA integrity is presented. In addition, non-enzymatic oviductal cell dissociation procedure is described. The methods are appropriate for subsequent gene and protein analysis of the functionally different oviductal segments and dissociated oviductal cells.

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Keywords: Microdissection Dissociation Murine Oviduct Segment Identification Single Cell Isolation Ampulla Infundibulum Stromal Cells Epithelial Cells Immune Cells Smooth Muscle Cells In Vitro Fertilization Malignant Transformation Tissue Dissection Tissue Dissociation
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