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Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers
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Chapters
- 00:06Introduction
- 02:01Day 1: Preparation of the 24-Well Plate
- 03:16Day 1: Cell Seeding
- 03:58Day 2: Addition of Test Drug
- 06:17Day 2: Imaging the Well-Plate
- 06:48Post-Experiment: Image Analysis
- 10:27Representative Results
- 12:22Discussion
This work presents a flexible protocol for utilizing fluorescently labeled elastomeric contractible surfaces (FLECS) Technology in microwell format for simplified, hands-off quantification of single-cell contractile forces based on visualized displacements of fluorescent protein micropatterns.
Tags
Keywords: Single-cell ContractilityMicropatterned ElastomersCell-generated Mechanical ForcesOrgan FunctionSmooth Muscle Cell ContractionContractile MechanismsCell Contractile FunctionDrug ScreeningMicrotechnologyFluorescently Labeled Elastomeric Contractable SurfacesProtein MicropatterningCell Position And ShapeCell Traction ForcesImage AnalysisCellular Force Biology
