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蓝藻中完整藻胆体的分离与表征
JoVE Journal
Biology
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JoVE Journal Biology
Isolation and Characterization of Intact Phycobilisome in Cyanobacteria
DOI:

06:26 min

November 10, 2021

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Chapters

  • 00:04Introduction
  • 00:38Cell Culture and Harvesting
  • 01:13Cells Lysis
  • 02:43Preparation of the Sucrose Gradient Buffers and PBS Isolation
  • 03:32Measurement of PBS Fluorescence at 77K
  • 04:00Results: Spectral Evaluation of Phycobilisomes
  • 05:50Conclusion

Summary

Automatic Translation

目前的方案详细介绍了通过不连续蔗糖密度梯度离心从蓝藻中分离藻胆体的过程。完整的藻糖体的分数通过77K荧光发射光谱和SDS-PAGE分析得到证实。所得的藻胆体组分适用于TEM的负染色和质谱分析。

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