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JoVE Journal Bioengineering
Fremstilling og anvendelse af tørre makroporøse alginatstilladser til viral transduktion af T-celler
 
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Fremstilling og anvendelse af tørre makroporøse alginatstilladser til viral transduktion af T-celler

Article DOI: 10.3791/64036-v • 11:16 min • September 9th, 2022
September 9th, 2022 •
1, 1,2, 1,2, 1,2,3
1Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, 2Comparative Medicine Institute, North Carolina State University, 3Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill

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Heri er en protokol til oprettelse af tørre makroporøse alginatstilladser, der formidler effektiv viral genoverførsel til brug i genteknologi af T-celler, herunder T-celler til CAR-T-celleterapi. Stilladserne viste sig at transducere aktiverede primære T-celler med >85% transduktion.

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Bioengineering udgave 187 biomateriale stillads alginat makroporøs viral transduktion immunterapi CAR-T-celler
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