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用荧光线粒体分离条件永生化的小鼠肾小球内皮细胞
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Biology
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JoVE Journal Biology
Isolation of Conditionally Immortalized Mouse Glomerular Endothelial Cells with Fluorescent Mitochondria
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用荧光线粒体分离条件永生化的小鼠肾小球内皮细胞

DOI:
10.3791/64147-v

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11:49 min

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September 13, 2022

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, , ,
1Department of Medicine, Division of Nephrology,The Icahn School of Medicine at Mount Sinai

Chapters

  • 00:04Introduction
  • 00:53Isolation of Mouse Kidney Glomerular Cells
  • 06:08Isolating the Glomerular Endothelial Cells with CD31‐Coated Beads
  • 09:04Results: Isolation and Characterization of MitoDendra2‐GECs
  • 11:04Conclusion

Summary

Automatic Translation

Automatic Translation

本文描述了从表达热不稳定猿病毒40和光激活线粒体PhAM切除的转基因小鼠的肾脏中分离条件永生化肾小球内皮细胞的方法。我们描述了使用磁珠、消化步骤、播种和培养 GEC-CD31 阳性从整个肾脏中分离肾小球的程序。

Tags

Immortalized Glomerular Endothelial CellsFluorescent MitochondriaIn VitroDiabetic Kidney DiseaseCell IsolationKidney Glomerular CellsMagnetic BeadsCollagen IVCollagenase Type IICell Culture

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