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Quantificazione sperimentale delle interazioni tra sistemi di somministrazione di farmaci e cellule in vitro: una guida per la valutazione preclinica della nanomedicina
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1Department of Microbiology and Immunology,The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, 2Department of Infectious Diseases,The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, 3Victorian Infectious Diseases Service,Royal Melbourne Hospital at the Peter Doherty Institute for Infection and Immunity, 4Department of Infectious Diseases,Alfred Hospital and Monash University, 5Department of Chemical Engineering,The University of Melbourne, 6Department of Biomedical Engineering,The University of Melbourne
Chapters
- 00:05Introduction
- 00:45Preparation
- 01:58Measurement
- 03:53Analysis
- 05:11Determination of the Absolute Fluorescence Intensity per Carrier
- 05:56Cell Experiment
- 07:08Results: A Two-Stream Methodology to Convert Relative, Semiquantitative Flow Cytometry Cell-Carrier Association Data into Absolute Quantification
- 08:17Conclusion
Viene dimostrato un flusso di lavoro per la quantificazione assoluta delle interazioni farmaco-cellula trasportatrice utilizzando la citometria a flusso per consentire una migliore valutazione razionale di nuovi sistemi di somministrazione di farmaci. Questo flusso di lavoro è applicabile ai vettori di droga di qualsiasi tipo.
