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在2D结构中模拟人类小脑体外发育

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Modeling Human Cerebellar Development In Vitro in 2D Structure

Please note that all translations are automatically generated. Click here for the English version.

在2D结构中模拟人类小脑体外发育

DOI:

10.3791/64462-v

•

06:14 min

•

September 16, 2022

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Deniz A. Madencioglu^3,4,5, Karina A. Kruth^3,4,5, Thomas H. Wassink³, Vincent A. Magnotta^2,3,4,5, John A. Wemmie^3,4,5, Aislinn J. Williams^3,4,5

¹Department of Psychiatry,University of Iowa, ²Department of Radiology,University of Iowa, ³Carver College of Medicine,University of Iowa, ⁴Iowa Neuroscience Institute,University of Iowa, ⁵Pappajohn Biomedical Institute,University of Iowa

Chapters

00:04Introduction
00:41Experimental Preparation
01:15Cerebellar Differentiation
03:59Results: Overview of the 3D to 2D Cerebellar Differentiation and Expression of Cerebellar Cell Markers
05:48Conclusion

Summary

Automatic Translation

本协议解释了从诱导多能干细胞生成2D单层小脑细胞，用于研究小脑发育的早期阶段。

Tags

2D Structure In Vitro Cerebellar Development Human Neuropsychiatric Disorders Cost-efficient Pulled Glass Pipette IPSC Embryoid Bodies Cerebellar Differentiation Medium FGF2

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