Journal
/
Immunology and Infection
/
全谱流式细胞术分析原代小鼠T细胞受体诱导的钙内流
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry
Please note that all translations are automatically generated. Click here for the English version.

全谱流式细胞术分析原代小鼠T细胞受体诱导的钙内流

DOI:
10.3791/64526-v

•

10:01 min

•

December 16, 2022

•

, ,
1Department of Immunology and Microbiology,University of Colorado-Anschutz School of Medicine

Chapters

  • 00:04Introduction
  • 00:33Indo-1 Ratiometric Dye and Fluorescent Antibody Labeling
  • 03:35Acquisition of Calcium Influx Using Flow Cytometric Analysis
  • 07:45Results: Visualization of Calcium Responses in Gated Populations of Murine Thymocytes
  • 09:17Conclusion

Summary

Automatic Translation

Automatic Translation

钙内流是T细胞信号传导的量度，是分析对T细胞受体刺激反应的有效方法。该协议将Indo-1与针对细胞表面分子的抗体组合多重化，利用了全光谱流式细胞术的高度灵活性。

Tags

T-cell ReceptorCalcium InfluxFlow CytometryIndo-1 AMCell StainingCell PhenotypingCell SubsetsEGTAIonomycinFc Receptor BlockingCell WashingMaster Mix Antibody Staining

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/64526?article_type=v

T细胞受体在小鼠T细胞的逆转录病毒转导
check_url/64526?article_type=v

流式细胞仪分析小鼠主动脉内流的免疫细胞
check_url/64526?article_type=v

piggyBac转座子初级人类T细胞系统改造
check_url/64526?article_type=v

检测荧光纳米粒子的相互作用与原发性免疫细胞亚群流式细胞仪的
check_url/64526?article_type=v

流式细胞仪用来评估染色质的T细胞国家
check_url/64526?article_type=v

使用成像流式细胞仪T细胞同种异体反应性测定
check_url/64526?article_type=v

小鼠与人肺泡巨噬细胞的分离和体外培养
check_url/64526?article_type=v

全血流式细胞术分析人单核细胞亚群的表征
check_url/64526?article_type=v

基于多色流式细胞仪的 t 细胞线粒体和溶酶体的定量
check_url/64526?article_type=v

流式细胞术测定小鼠胸腺、胰腺干淋巴结和脾脏的调节 t 细胞亚群

Read Article