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1Animal and Veterinary Research Centre (CECAV), Associate Laboratory for Animal and Veterinary Science-AL4AnimalS,University of Trás-os-Montes and Alto Douro (UTAD), 2Pathology Laboratory,UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Oeiras, 3Pathology Laboratory,UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Vairão
Chapters
- 00:04Introduction
- 00:48Deparaffinization and Rehydration
- 01:39Epitope Retrieval
- 02:31Inactivation of Endogenous Peroxidase and Immunodetection
- 04:59Results: Epitope Demasking for Proper PrP Immunolabeling and Minimizing Non-Specific Background Staining
- 06:00Conclusion
Immunolabeling abnormal prion protein using immunohistochemistry protocols requires specific sample and anti-PrP antibody preparation methodologies. The present protocol describes the key steps in epitope demasking to ensure proper PrP immunolabeling and to minimize non-specific background staining. Also, this approach considers biosafety measures when conducting immunohistochemistry studies with the prion-infected tissues.
