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免疫组化检测异常朊蛋白
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Detection of Abnormal Prion Protein by Immunohistochemistry
DOI:

06:38 min

May 05, 2023

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Chapters

  • 00:04Introduction
  • 00:48Deparaffinization and Rehydration
  • 01:39Epitope Retrieval
  • 02:31Inactivation of Endogenous Peroxidase and Immunodetection
  • 04:59Results: Epitope Demasking for Proper PrP Immunolabeling and Minimizing Non-Specific Background Staining
  • 06:00Conclusion

Summary

Automatic Translation

使用免疫组织化学方案对异常朊蛋白进行免疫标记需要特定的样品和抗PrP抗体制备方法。本协议描述了表位去掩膜的关键步骤,以确保适当的PrP免疫标记并最大限度地减少非特异性背景染色。此外,这种方法在对朊病毒感染的组织进行免疫组织化学研究时考虑了生物安全措施。

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