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Adoptiver Transfer von IL-33-stimulierten Makrophagen in Bleomycin-induzierte Mausmodelle, um ihre Wirkung auf die idiopathische Lungenfibrose in vivo zu untersuchen
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo
DOI:

06:29 min

May 05, 2023

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Chapters

  • 00:04Introduction
  • 00:39Preparation of Recipient Mouse
  • 01:13Isolation and Culture of Interstitial Macrophages (IMs)
  • 03:13Adoptive Transfer of IMs into the Lung Alveoli
  • 05:12Results: Effect of IL‐33‐Stimulated Macrophages on Idiopathic Pulmonary Fibrosis
  • 05:59Conclusion

Summary

Automatic Translation

Dieses Protokoll beschreibt die Isolierung von pulmonalen interstitiellen Makrophagen (IMs) und deren adoptiven Transfer nach IL-33-Stimulation der Lungenbläschen in einem Mausmodell, was die In-vivo-Untersuchung der idiopathischen Lungenfibrose (IPF) erleichtern kann.

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