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Neuroscience
/
大鼠背根神经节外植体和雪旺细胞共培养中外周轴突的体外髓鞘形成
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells
Please note that all translations are automatically generated.
Click here for the English version.
大鼠背根神经节外植体和雪旺细胞共培养中外周轴突的体外髓鞘形成
DOI:
10.3791/64768-v
•
08:57 min
•
February 10, 2023
•
Alina Blusch
,
Melissa Sgodzai
,
Niklas Rilke
,
Jeremias Motte
,
Jennifer König
,
Kalliopi Pitarokoili
,
Thomas Grüter
1
Department of Neurology
,
Ruhr-University Bochum, St. Josef Hospital
Chapters
00:05
Introduction
00:49
Sciatic Nerve Preparation and Refurbishment
02:18
Enzymatic Digestion of the Sciatic Nerve
03:04
Cell Separation
03:59
Schwann Cell Magnetic Separation
04:56
Dorsal Root Ganglia (DRG) Preparation
06:04
DRG Cell Culture and Coculture with Schwann Cells
07:13
Results: Myelination in Coculture of DRG Explants and Schwann Cells
08:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在背根神经节和雪旺细胞的共培养系统中,可以研究周围神经系统的髓鞘形成。该模型为观察和量化外周髓鞘形成以及研究目标化合物对髓鞘的影响提供了实验机会。
Tags
In Vitro Myelination
Peripheral Axons
Dorsal Root Ganglion Explants
Schwann Cells
Peripheral Nervous System
Axon-Schwann Cell Interactions
Myelination Quantification
DRG Explant Cultures
Neurodegenerative Diseases
Peripheral Nerve System
Sciatic Nerve
Nerve Fibers
Enzymatic Digestion
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