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Developmental Biology
/
人卵泡中的基因表达分析
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Gene Expression Analyses in Human Follicles
Please note that all translations are automatically generated.
Click here for the English version.
人卵泡中的基因表达分析
DOI:
10.3791/64807-v
•
09:09 min
•
February 17, 2023
•
Melody Devos*
1
,
Joana Dias Nunes*
1
,
Isabelle Demeestere
2
1
Research Laboratory on Human Reproduction
,
Université libre de Bruxelles (ULB)
,
2
Fertility Clinic, Erasme Hospital
,
Université libre de Bruxelles (ULB)
Chapters
00:05
Introduction
00:54
Thawing of Cryopreserved Ovarian Tissue
01:58
Follicle Isolation
04:26
RNA Extraction
07:01
Results: Isolation and Gene Expression Analyses in Human Ovarian Follicles
08:16
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了一个协议,概述了如何从冷冻解冻的皮质组织中分离人类卵巢卵泡以进行基因表达分析。
Tags
Gene Expression
Ovarian Follicles
Germ Cells
Gene Expression Analysis
Follicle Isolation
Enzymatic Isolation
Mechanical Isolation
Ovarian Cortical Fragment
Cryoprotectant Solution
Leibovitz 15 Medium
Dissection Medium
Digestion Medium
Scalpel
Petri Dish
Liquid Nitrogen
RT
Carbon Dioxide
Incubator
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