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人隐静脉内皮细胞分离和暴露于受控水平的剪切应力和拉伸
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Medicine
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JoVE Journal
Medicine
Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch
Please note that all translations are automatically generated.
Click here for the English version.
人隐静脉内皮细胞分离和暴露于受控水平的剪切应力和拉伸
DOI:
10.3791/65122-v
•
09:10 min
•
April 21, 2023
•
Thaís Girão-Silva*
1,2
,
Miriam Helena Fonseca-Alaniz*
1
,
Luís Alberto Oliveira Dallan
,
Iuri Cordeiro Valãdao
,
Gustavo Henrique Oliveira da Rocha
,
José Eduardo Krieger
,
Ayumi Aurea Miyakawa
1
Instituto do Coraçao (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina
,
Universidade de Sao Paulo
,
2
Cardiology Research Group, Faculty VI Medicine and Health Sciences
,
Carl von Ossietzky University of Oldenburg
Chapters
00:04
Introduction
01:18
Isolation and Culture of Primary Human Saphenous Vein Endothelial Cells (hSVECs)
03:37
Applying Shear Stress on Human SVECs
05:42
Applying Cyclic Stretch on Human SVECs
07:20
Results: Effects of Mechanical Stress in Human SVECs
08:34
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们描述了一种分离和培养人隐静脉内皮细胞(hSVEC)的方案。我们还提供了产生剪切应力和拉伸的详细方法,以研究hSVEC中的机械应力。
Tags
Saphenous Vein
Endothelial Cell Isolation
Shear Stress
Stretch
Coronary Artery Bypass Surgery
Mechanical Stress
Cell Culture
Collagenase
Smooth Muscle Cells
Fibroblasts
Growth Factors
PBS
Cell Medium
Centrifugation
Cell Pellet
Gelatin Coating
Cell Culture Dish
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