Journal
/
Medicine
/
人隐静脉内皮细胞分离和暴露于受控水平的剪切应力和拉伸
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch
Please note that all translations are automatically generated. Click here for the English version.

人隐静脉内皮细胞分离和暴露于受控水平的剪切应力和拉伸

DOI:
10.3791/65122-v

•

09:10 min

•

April 21, 2023

•

, , , , , ,
1Instituto do Coraçao (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina,Universidade de Sao Paulo, 2Cardiology Research Group, Faculty VI Medicine and Health Sciences,Carl von Ossietzky University of Oldenburg

Chapters

  • 00:04Introduction
  • 01:18Isolation and Culture of Primary Human Saphenous Vein Endothelial Cells (hSVECs)
  • 03:37Applying Shear Stress on Human SVECs
  • 05:42Applying Cyclic Stretch on Human SVECs
  • 07:20Results: Effects of Mechanical Stress in Human SVECs
  • 08:34Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一种分离和培养人隐静脉内皮细胞（hSVEC）的方案。我们还提供了产生剪切应力和拉伸的详细方法，以研究hSVEC中的机械应力。

Tags

Saphenous VeinEndothelial Cell IsolationShear StressStretchCoronary Artery Bypass SurgeryMechanical StressCell CultureCollagenaseSmooth Muscle CellsFibroblastsGrowth FactorsPBSCell MediumCentrifugationCell PelletGelatin CoatingCell Culture Dish

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

小鼠呼吸道上皮细胞和实验香烟烟雾暴露在空气分离液界面

植入小鼠触发剪应力致动脉粥样硬化的颈袖

树脂浇铸和3维成像的小鼠门静脉或肝内胆管系统

控制宫颈裂伤损伤小鼠

分离，培养和成像胎儿胰腺细胞集群

一种改进<em>在体外</em>入侵检测，以确定激素，在调节肿瘤细胞侵袭的细胞因子和/或生长因子的潜在作用

步骤人类隐静脉<em>前体内</em>灌注和外部强化

人淋巴管内皮细胞中的多参​​数荧光激活细胞分选分离

的肿瘤特异性内皮细胞的分离与培养扩展

分离培养犬从动脉和静脉主血管内皮细胞

Read Article