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Vena safena umana Isolamento delle cellule endoteliali ed esposizione a livelli controllati di stress da taglio e allungamento
JoVE Journal
Medicine
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JoVE Journal Medicine
Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch
DOI:

09:10 min

April 21, 2023

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Chapters

  • 00:04Introduction
  • 01:18Isolation and Culture of Primary Human Saphenous Vein Endothelial Cells (hSVECs)
  • 03:37Applying Shear Stress on Human SVECs
  • 05:42Applying Cyclic Stretch on Human SVECs
  • 07:20Results: Effects of Mechanical Stress in Human SVECs
  • 08:34Conclusion

Summary

Automatic Translation

Descriviamo un protocollo per isolare e coltivare cellule endoteliali della vena safena umana (hSVEC). Forniamo anche metodi dettagliati per produrre stress di taglio e allungamento per studiare lo stress meccanico nelle hSVEC.

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