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Journal / Biochemistry / LERLIC-MS / MS for Indgående Karakterisering og…
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Biochemistry

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JoVE Journal Biochemistry
LERLIC-MS / MS for Indgående Karakterisering og Kvantificering af glutamin og asparagin deamidering i Shotgun Proteomics
Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
Journal (Biochemistry)
Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors
Journal (Biochemistry)
Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors
 
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LERLIC-MS / MS for Indgående Karakterisering og Kvantificering af glutamin og asparagin deamidering i Shotgun Proteomics

Article DOI: 10.3791/55626-v • 08:01 min • April 9th, 2017
April 9th, 2017 •
*1, *1, 1
1Division of Structural Biology and Biochemistry, School of Biological Sciences, Nanyang Technological University
* These authors contributed equally

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Her præsenterer vi en trin-for-trin-protokollen af ​​den lange længde elektrostatisk frastødning-hydrofil interaktionskromatografi-tandem massespektrometri (LERLIC-MS / MS) metode. Dette er en hidtil ukendt metode, som gør det muligt for første gang kvantificering og karakterisering af glutamin og asparagin deamideringssteder isoformer med shotgun proteomics.

Tags

Biokemi Deamidering glutamin asparagin ERLIC LERLIC Degenerative proteinmodifikationer DPM'er posttranslationelle modifikationer PTMS transamidering Long-længde anionbytter-LAX Shotgun proteomics HILIC.
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