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Journal / Medicine / Flödescytometrianalys av immuncellsdelmängder…
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Flödescytometrianalys av immuncellsdelmängder inom Murine Mjälte, Benmärg, Lymfkörtlar och synovial vävnad i en artrosmodell
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Flödescytometrianalys av immuncellsdelmängder inom Murine Mjälte, Benmärg, Lymfkörtlar och synovial vävnad i en artrosmodell

Article DOI: 10.3791/61008-v • 12:23 min • April 24th, 2020
April 24th, 2020 •
1,2, 1,3, 1, 1, 1, 1
1Raymond Purves Bone and Joint Research Laboratory, Institute of Bone and Joint Research, Kolling Institute, Royal North Shore Hospital, University of Sydney, 2HTRG - Heidelberg Trauma Research Group, Center for Orthopedics, Trauma Surgery and Spinal Cord Injury, Trauma and Reconstructive Surgery, Heidelberg University Hospital, 3Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University

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Här beskriver vi en detaljerad och reproducerbara flöde cytometry protokollet för att identifiera monocyte/macrophage och T-cells delmängder med hjälp av både extra- och intracellulära färgning assays inom murine mjälte, benmärg, lymfkörtlar och synovial vävnad, utnyttja en etablerad kirurgisk modell av murine artros.

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Medicin Artros immunologi flödescytometri lymfocytundergrupper T-celler monocyter makrofager ortopedi posttraumatisk artros
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