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Bir Osteoartrit Modelinde Minör Dalak, Kemik İliği, Lenf Düğümleri ve Sinovyal DokuDaki İmmün Hücre Alt Kümelerinin Akış Sitometri Analizi
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Bir Osteoartrit Modelinde Minör Dalak, Kemik İliği, Lenf Düğümleri ve Sinovyal DokuDaki İmmün Hücre Alt Kümelerinin Akış Sitometri Analizi

Article DOI: 10.3791/61008-v • 12:23 min • April 24th, 2020
April 24th, 2020 •
1,2, 1,3, 1, 1, 1, 1
1Raymond Purves Bone and Joint Research Laboratory, Institute of Bone and Joint Research, Kolling Institute, Royal North Shore Hospital, University of Sydney, 2HTRG - Heidelberg Trauma Research Group, Center for Orthopedics, Trauma Surgery and Spinal Cord Injury, Trauma and Reconstructive Surgery, Heidelberg University Hospital, 3Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University

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Burada, murine dalak, kemik iliği, lenf düğümleri ve sinovyal doku içinde hem ekstra hem de hücre içi boyama tahlilleri kullanarak monosit/makrofaj ve T hücrealt kümelerini tanımlamak için ayrıntılı ve tekrarlanabilir akış sitometri protokolünü tanımladık ve murine osteoartritin yerleşik bir cerrahi modelini kullanıyoruz.

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Tıp Sayı 158 Osteoartrit immünoloji akış sitometrisi lenfosit alt kümeleri T-hücreleri monositler makrofajlar ortopedik posttravmatik osteoartrit
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