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Journal / Medicine / Flow Cytometri Analyse af immuncelledelmængder i…
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Flow Cytometri Analyse af immuncelledelmængder i Murine Milten, Knoglemarv, Lymfeknuder og synovialvæv i en osteokhritis model
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Flow Cytometri Analyse af immuncelledelmængder i Murine Milten, Knoglemarv, Lymfeknuder og synovialvæv i en osteokhritis model

Article DOI: 10.3791/61008-v • 12:23 min • April 24th, 2020
April 24th, 2020 •
1,2, 1,3, 1, 1, 1, 1
1Raymond Purves Bone and Joint Research Laboratory, Institute of Bone and Joint Research, Kolling Institute, Royal North Shore Hospital, University of Sydney, 2HTRG - Heidelberg Trauma Research Group, Center for Orthopedics, Trauma Surgery and Spinal Cord Injury, Trauma and Reconstructive Surgery, Heidelberg University Hospital, 3Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University

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Her beskriver vi en detaljeret og reproducerbar flow cytometri protokol til at identificere monocyt / makrofag og T-celle delmængder ved hjælp af både ekstra- og intracellulære farvning assays inden for murine milten, knoglemarv, lymfeknuder og synovialvæv, ved hjælp af en etableret kirurgisk model af murin slidgigt.

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Medicin Slidgigt immunologi flow cytometri lymfocyt delmængder T-celler monocytter makrofager ortopædi posttraumatisk slidgigt
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