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JoVE Journal Biology
Interrogazione accoppiata cellula-specifica dell'epigenoma ovarico e del trascrittoma ovarico del topo
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Interrogazione accoppiata cellula-specifica dell'epigenoma ovarico e del trascrittoma ovarico del topo

Article DOI: 10.3791/64765-v • 12:25 min • February 24th, 2023
February 24th, 2023 •
*1,2,3, *1,3, 1, 2, 2, 4, 2,3, 1,3
1Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, 2Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, 3Oklahoma City Veterans Affairs Medical Center, 4Nutrition College, Federal University of Pelotas
* These authors contributed equally

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In questo protocollo, il metodo di purificazione dell'affinità dei ribosomi traslanti (TRAP) e l'isolamento dei nuclei marcati in specifici tipi cellulari (INTACT) sono stati ottimizzati per l'interrogazione accoppiata del trascrittoma ovarico e dell'epigenoma specifici della cellula utilizzando il modello murino NuTRAP incrociato su una linea murina Cyp17a1-Cre.

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Questo mese in JoVE numero 192
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