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JoVE Journal Biology
Cellspecifik parad förhör av musens äggstocksepigenom och transkriptom
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Cellspecifik parad förhör av musens äggstocksepigenom och transkriptom

Article DOI: 10.3791/64765-v • 12:25 min • February 24th, 2023
February 24th, 2023 •
*1,2,3, *1,3, 1, 2, 2, 4, 2,3, 1,3
1Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, 2Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, 3Oklahoma City Veterans Affairs Medical Center, 4Nutrition College, Federal University of Pelotas
* These authors contributed equally

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I detta protokoll optimerades den översättande ribosomaffinitetsreningsmetoden (TRAP) och isoleringen av kärnor taggade i specifika celltyper (INTACT) -metoden för den parade undersökningen av det cellspecifika äggstockstranskriptomet och epigenomet med hjälp av NuTRAP-musmodellen korsad till en Cyp17a1-Cre-muslinje.

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Denna månad i JoVE nummer 192
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