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Cellespesifikt parret avhør av musens ovarieepigenom og transkriptom
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Cellespesifikt parret avhør av musens ovarieepigenom og transkriptom

Article DOI: 10.3791/64765-v • 12:25 min • February 24th, 2023
February 24th, 2023 •
*1,2,3, *1,3, 1, 2, 2, 4, 2,3, 1,3
1Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, 2Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, 3Oklahoma City Veterans Affairs Medical Center, 4Nutrition College, Federal University of Pelotas
* These authors contributed equally

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I denne protokollen ble metoden for translatering av ribosomaffinitetsrensing (TRAP) og isolering av kjerner merket i spesifikke celletyper (INTAKT) optimalisert for parret undersøkelse av det cellespesifikke ovarietranskriptomet og epigenomet ved bruk av NuTRAP-musemodellen krysset til en Cyp17a1-Cre muselinje.

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