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单核RNA-seq和ATAC-seq的新鲜冷冻脑肿瘤的核隔离
JoVE 杂志
癌症研究
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JoVE 杂志 癌症研究
Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
DOI:

06:22 min

August 25, 2020

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Chapters

  • 00:04Introduction
  • 01:01Tissue Dissection and Dissociation
  • 03:20Gradient Centrifugation and Isolation of Nuclei
  • 04:56Results: Comparison of UMIs and Genes Obtained from Different Human Brain snRNA-seq Datasets
  • 05:57Conclusion

Summary

自动翻译

肿瘤内异质性是肿瘤的内在特征,包括胶质瘤。我们开发了一个简单而高效的协议,利用缓冲和梯度离心的组合,将单核从新鲜冷冻胶质瘤组织中分离出来,用于单核RNA和ATAC测序研究。

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