İndüksiyon ve İnsanlarda Egzersizde İskelet Kası Hasar değerlendirilmesi
Summary
This article describes a safe and reliable method to induce and quantify exertional skeletal muscle damage in human subjects.
Abstract
gönüllü eksantrik (uzatma) kasılmalar yoluyla daralma bağlı kas hasarı insanlarda kas adaptasyon ve kurtarma eğitimi için mükemmel bir model sunuyor. Bu yazıda gücü, ağrı ve plazma kreatin kinaz düzeylerindeki değişikliklerle işaretlenmiş kuadriseps kasları, hasar uyarılması için bir eksantrik egzersiz protokol tasarım tartışır. insan katılımcıların gerçekleştirilen ve sonuçları türler arası çeviri ortadan kaldırır, çünkü bu yöntem, basit, etik ve yaygın olarak uygulanmaktadır. Denekler bir hızda diz ekstansör kaslarının 300 maksimal eksantrik kasılmaları gerçekleştirmek 120 ° / izokinetik dinamometre üzerinde sn. Hasarın büyüklüğü egzersiz sonrası birkaç gün içinde mukavemet kaybı, ağrı ve plazma kreatinin kinaz düzeylerinin göreceli olarak non-invaziv izokinetik ve izometrik ölçümler kullanılarak ölçülebilir. Bu nedenle, uygulama kas mekanizmalarını belirlemek amacıyla belirli nüfusa yönelik olabiliradaptasyon ve yenilenme.
Introduction
The overall goal of this procedure is to induce exertional damage to the quadriceps femoris muscles using voluntary lengthening (eccentric) contractions in human subjects.
Contraction-induced skeletal muscle damage is a common consequence of exercise that is marked by delayed onset muscle soreness1, transient strength loss, and elevated muscle-specific enzymes in the blood2. Exertional muscle damage is most pronounced following exercise to which the subject is unaccustomed, particularly when eccentric contractions are involved3. Exertional muscle damage is typically benign. Soreness subsides, and both serum proteins and strength typically return to pre-damage levels within a few days to weeks after the damaging insult. In extreme cases, exertional muscle damage can lead to a life-threatening syndrome know as rhabdomyolysis. However, exertional muscle damage is usually insufficient to cause clinical rhabdomyolysis in healthy individuals4 in the absence of compounding factors including heat stress, dehydration5, infection6 or rare genetic predispositions7.
Contraction-induced muscle damage is typically less severe than toxin-induced or freezing-induced injury, methods often used in rodent studies8,9. Yet, contraction-induced injury provides a useful method to study the muscle damage response with notable advantages. First, it is a safe and ethical method for use with human subjects1-3. Thus, interspecies translation of the results is not needed as data can be obtained directly from human subjects. Moreover, translating data obtained from rodent studies is very difficult given that the severity of injury seen in the rodent injury models exceeds the level of damage that would be ethical to induce in human subjects. Second, contraction-induced damage is commonly experienced and a natural process of exercise. Therefore, this mode of damage induction is useful for studying muscle damage in the context of exercise, adaptation to exercise as well as overt muscle injury. Here we describe a safe and reliable method to induce and evaluate skeletal muscle damage using lengthening contractions in humans.
Protocol
Representative Results
Discussion
Birkaç adım bu protokolün istenen sonuçları elde etmek için kritik öneme sahiptir. İlk olarak, deneklere uygun daralma protokolleri, özellikle kuvvet ölçümleri aşina olmalıdır. konu yaptıklarını ve onlara veri toplama öncesinde kuvvet uygulama testleri için bir fırsat vermek beklenen tam olarak ne anladığından emin olun. yeterince bu işlemler aşina olmayan konular zarar indüksiyon şu günlerde üzerinde bir öğrenme eğrisi gösterebilir. Bu geçersiz gücü ölçümlerini render bir karış…
Divulgaciones
The authors have nothing to disclose.
Acknowledgements
The authors have no acknowledgements.
Materials
| Biodex Dynomometer | Biodex Medical Systems | 850-000 | Other models are available and should produce similar results |
| Creatine Kinase kit | Sigma-Aldrich | MAK116 | |
| Serum Vacutainers | BD Bioscience | 367812 | |
| Winged safety push button blood collection set | BD Bioscience | 367338 | |
| Cryogenic vials | Sigma-Aldrich | V5007 | We use the 2mL vials to store serum aliquots |
Referencias
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