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Capítulos
- 00:05Título
- 00:39Cell Culture for Immunofluorescence Microscopy
- 01:32Preparation of Transfection Complex and Transfection of Cells
- 03:08Live Cell Imaging and Indirect Immunofluorescence
- 05:40Results: Identifying Kinesin Cargo via Fluorescence Microscopy
- 06:50Conclusion
Tags
Kinesin-1CargosFluorescence MicroscopyMolecular MotorsDominant Negative KIF5B MutantsLive Cell ImagingIndirect Fluorescence ImagingImmunofluorescent StudiesCover GlassesCell CultureTransfection MediumTdTomato-tagged Wild Type KIF5B Expression PlasmidMotorless KIF5B Expression PlasmidCandidate Cargo Protein
