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Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging
 

Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging

Article DOI: 10.3791/1389-v • 16:44 min • June 2nd, 2009
June 2nd, 2009 •
1,2, 3, 1,2, 1,2, 1,2, 1,2, 3, 1,2
1Center for Systems Biology, Harvard Medical School, 2Center for Systems Biology, MGH - Massachusetts General Hospital, 3Institute for Biological and Medical Imaging, Technical University of Munich and Helmholtz Center Munich

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We suggest a Born normalized approach for Optical Projection Tomography (BnOPT) that accounts for the absorption properties of imaged samples to obtain accurate and quantitative fluorescence tomographic reconstructions. We use the proposed algorithm to reconstruct the fluorescence molecular probe distribution within small animal organs.

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Born Normalization Fluorescence Optical Projection Tomography Whole Heart Imaging Three-dimensional Imaging Technique Developmental Biology Gene Expression Studies Optical Transparency Benzyl Alcohol Benzyl Benzoate BABB Murray's Clear Solution Clearing Process Scattering Contribution Absorption Contribution Fluorescence Distribution Image Artifacts Quantification Errors Exogenous Contrast Agents Endogenous Contrast
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