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JoVE Journal Neuroscience
Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue
 
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Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue

Article DOI: 10.3791/2681-v • 07:29 min • May 25th, 2011
May 25th, 2011 •
1, 2, 3, 4, 1
1Department of Neurology, Beth Israel Deaconess Medical Center, 2Department of Obstetrics and Gynecology, Brigham and Women's Hospital, 3Department of Pathology, Beth Israel Deaconess Medical Center, 4Department of Pathology, Division of Neuropathology, Brigham and Women's Hospital

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A simple and reliable method on isolation and culture of neural stem cells from discarded human fetal cortical tissue is described. Cultures derived from known human neurological disorders can be used for characterization of pathological cellular and molecular processes, as well as provide a platform to assess pharmacological efficacy.

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Neural Stem Cells NSCs Cortical Tissue Fetal Tissue Neuroepithelium Cortical Plate Intermediate Progenitors Neuronal Cells Glial Cells Cerebral Cortex Normal Human NSC Development Neurological Disorders Progenitor Proliferation Migration Differentiation Down Syndrome NSCs Alzheimer's Disease Phenotype Genes Located On Human Chromosome 21 Harvesting Tissue Dissection Cell Sorting Plating Passaging Of Human NSCs Differentiation Of Human NSCs
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