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Bioengineering

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JoVE Journal Bioengineering
Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells (hESC) to Different Lineages
 

Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells (hESC) to Different Lineages

Article DOI: 10.3791/3608-v • 10:02 min • March 9th, 2012
March 9th, 2012 •
1, 1, 2, 1, 3
1Stem Cell Lab, School of Psychiatry, Faculty of Medicine, The University of New South Wales, 2Siriraj Center of Excellence for Stem cell Research, Faculty of Medicine Siriraj Hospital, Mahidol University, 3Neuropsychiatric Institute, Prince of Wales Hospital

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We have optimized a microencapsulation technique as an effective 3D platform for propagation and differentiation of embryonic stem cells to endoderm and dopaminergic (DA) neurons. It also provides an opportunity for immune-isolation of cells from the host during transplantation. This platform can be adapted for other cell types.

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Alginate Microcapsule 3D Platform Propagation Differentiation Human Embryonic Stem Cells HESC Biomaterials Stem Cell Cultures Microenvironment Cell-to-cell Interaction Cell Proliferation Specific Lineages Tissue Organization Encapsulation Semi-permeable Membranes 3D Cultures Nutrients Exchange Oxygen Exchange Stimuli Exchange Antibodies Exclusion Immune Cells Exclusion Alginate Microcapsules Culture Conditions Enhancement P160-Rho-associated Coiled-coil Kinase Inhibitor ROCK Inhibitor Y-27632 Human Fetal Fibroblast-conditioned Serum Replacement Medium
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