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JoVE Journal Bioengineering
Layered Alginate Constructs
 

Layered Alginate Constructs: A Platform for Co-culture of Heterogeneous Cell Populations

Article DOI: 10.3791/54380-v • 08:57 min • August 7th, 2016
August 7th, 2016 •
1, 1, 1, 1
1Fischell Department of Bioengineering, University of Maryland

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Engineering and analysis of load bearing tissues with heterogeneous cell populations are still a challenge. Here, we describe a method for creating bi-layered alginate hydrogel discs as a platform for co-culture of diverse cell populations within one construct.

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Keywords: Alginate Hydrogel Bi-layered Construct Co-culture Heterogeneous Cell Populations 3D Culture Tissue Engineering Mechanical Stimulation Cell Microsieve Membrane Gel Formation Mold Cell Suspension Cell Seeding
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