Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Bioengineering

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

Generation and Recovery of β-cell Spheroids From Step-growth PEG-peptide Hydrogels
 

Generation and Recovery of β-cell Spheroids From Step-growth PEG-peptide Hydrogels

Article DOI: 10.3791/50081-v 09:21 min December 6th, 2012
December 6th, 2012

Chapitres

résumé

The following protocol provides techniques for encapsulating pancreatic β-cells in step-growth PEG-peptide hydrogels formed by thiol-ene photo-click reactions. This material platform not only offers a cytocompatible microenvironment for cell encapsulation, but also permits user-controlled rapid recovery of cell structures formed within the hydrogels.

Tags

Keywords: PEG Hydrogels Pancreatic Beta-cells MIN6 Cells Thiol-ene Crosslinking Chymotrypsin-sensitive Peptide 3D Cell Culture Cell Encapsulation Cell Viability Cell Spheroid Recovery Tissue Engineering
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter