Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Electroporation of the Hindbrain to Trace Axonal…
JoVE Journal
Neuroscience

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Neuroscience
Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo
 

Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo

Article DOI: 10.3791/50136-v • 10:04 min • May 29th, 2013
May 29th, 2013 •
1, 2, 2, 1
1Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, 2Department of Medical Neurobiology, The Hebrew University of Jerusalem

Chapitres

résumé
Automatic Translation

How neuronal networks are established in the embryonic brain is a fundamental question in developmental neurobiology. Here we combined an electroporation technique with novel genetic tools, such as Cre/Lox–plasmids and PiggyBac-mediated DNA transposition system in the avian hindbrain to label dorsal interneurons and track their axonal projections and synaptic targets at various developmental stages.

Tags

Keywords: Electroporation Hindbrain Chick Embryo Axonal Trajectory Synaptic Target Neural Tube Cre/Lox PiggyBac GFP Interneurons DA1 Brainstem
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter