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RNA Secondary Structure Prediction Using High-throughput SHAPE
 
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RNA Secondary Structure Prediction Using High-throughput SHAPE

Article DOI: 10.3791/50243-v • 13:42 min • May 31st, 2013
May 31st, 2013 •
*1, *1, 1, 1, 1
1RT Biochemistry Section, HIV Drug Resistance Program, Frederick National Laboratory for Cancer Research
* These authors contributed equally

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High-throughput selective 2' hydroxyl acylation analyzed by primer extension (SHAPE) utilizes a novel chemical probing technology, reverse transcription, capillary electrophoresis and secondary structure prediction software to determine the structures of RNAs from several hundred to several thousand nucleotides at single nucleotide resolution.

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RNA Secondary Structure Prediction High-throughput SHAPE RNA Structure Selective 2' Hydroxyl Acylation Analyzed By Primer Extension SHAPE Reagents Chemical Probing Agents Reverse Transcription Capillary Electrophoresis (CE) CDNA Library ShapeFinder Software Nucleotide Reactivity Tables Pseudo-energy Constraints RNAStructure Prediction Algorithm Two-dimensional RNA Structures
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