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Immunology and Infection

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JoVE Journal Immunology and Infection
Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR
 

Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

Article DOI: 10.3791/52184-v • 14:14 min • December 6th, 2014
December 6th, 2014 •
1, 1, 1, 1, 1, 1, 1
1CREA, Diagnostics Department, Spedali Civili di Brescia

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Here, we describe a method for simultaneous quantification of T-cell receptor excision circles (TRECs) and K-deleting recombination excision circles (KRECs). The TREC/KREC assay can be used as marker of thymic and bone marrow output.

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T-cell Receptor Excision Circles TRECs K-deleting Recombination Excision Circles KRECs Real-time PCR Quantification DNA Elements Recombination Process T-cell Receptors B-cell Receptors Peripheral Blood Estimation Thymic Output Bone Marrow Output Duplex Quantitative Real-time PCR T Lymphocytes B Lymphocytes Standard Curve Bacterial Plasmid Reference Gene Immune Reconstitution Bone Marrow Transplantation Immune Deficiencies Immunomodulating Drug Activity
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