Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / High Throughput Fluorometric Technique for…
JoVE Journal
Immunology and Infection

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Immunology and Infection
High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization
 

High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization

Article DOI: 10.3791/52195-v • 09:22 min • November 27th, 2014
November 27th, 2014 •
1,3, 1,2
1Department of Pharmacology, University of Minnesota, 2Department of Surgery, University of Minnesota, 33M Corporate Research Laboratory

Chapitres

résumé
Automatic Translation

Here we present a protocol to quantify phagocytosis of fluorescent particles by adherent macrophage cell line using a fluorometric method. This method facilitates a high throughput quantification of particle internalization as well as the resulting actin polymerization.

Tags

Keywords: Fluorometric Analysis Phagocytosis Actin Polymerization High Throughput Macrophages Fluorescent Particles Trypan Blue DAPI Flow Cytometry Cellular Processes
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter