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JoVE Journal
Immunology and Infection

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In Vivo Assessment of Alveolar Macrophage Efferocytosis Following Ozone Exposure
 

In Vivo Assessment of Alveolar Macrophage Efferocytosis Following Ozone Exposure

Article DOI: 10.3791/60109-v 08:54 min October 22nd, 2019
October 22nd, 2019

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résumé

This manuscript describes a protocol for determining whether exposure to ozone, a criteria air pollutant, impairs alveolar macrophage efferocytosis in vivo. This protocol utilizes commonly used reagents and techniques and can be adapted to multiple models of pulmonary injury to determine effects on alveolar macrophage efferocytosis.

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Keywords: Alveolar Macrophage Efferocytosis Ozone Exposure Lung Injury Inflammation Jurkat T Cells Apoptotic Cells UV Irradiation Cell Culture In Vivo Assessment
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