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JoVE Journal Neuroscience
Enrichment of Bruch's Membrane from Human Donor Eyes
 

Enrichment of Bruch's Membrane from Human Donor Eyes

Article DOI: 10.3791/53382-v • 10:22 min • November 15th, 2015
November 15th, 2015 •
1,2, 1,2, 1,2, 1,2
1Centre for Ophthalmology & Vision Sciences, Institute of Human Development, University of Manchester, 2Centre for Advanced Discovery and Experimental Therapeutics, Central Manchester University Hospitals NHS Foundation Trust, Manchester Academic Health Science Centre

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résumé
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Identifying proteins specifically associated with Bruch’s membrane in human eyes is an important step in understanding the biochemical mechanisms behind eye diseases such as age-related macular degeneration. This protocol describes how to enrich this sheet of extracellular matrix for down-stream biochemical analysis.

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Bruch's Membrane Human Donor Eyes Age-related Macular Degeneration (AMD) Visual Impairment Retina Macula Central Vision Loss Soft Drusen Geographic Atrophy Neovascularisation Genetic Influences Biochemical Consequences Genetic Risk Bruch's Membrane Biochemistry Extracellular Matrix Retinal Pigment Epithelial (RPE) Cell Monolayer Drusen Formation Nutrient Flow Disruption Protein Composition
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