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Journal / Genetics / Detection of Copy Number Alterations Using Single …
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Genetics

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JoVE Journal Genetics
Detection of Copy Number Alterations Using Single Cell Sequencing
 

Detection of Copy Number Alterations Using Single Cell Sequencing

Article DOI: 10.3791/55143-v • 09:45 min • February 17th, 2017
February 17th, 2017 •
1,2,3, 4, 5
1Koch Institute for Integrative Cancer Research, Department of Biology, Massachusetts Institute of Technology, 2Howard Hughes Medical Institute, 3Division of Health Sciences and Technology, Harvard Medical School, 4The Barbara K. Ostrom (1978) Bioinformatics and Computing Facility in the Swanson Biotechnology Center, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 5BioMicro Center, Department of Biology, Massachusetts Institute of Technology

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Single cell sequencing is an increasingly popular and accessible tool for addressing genomic changes at high resolution. We provide a protocol that uses single cell sequencing to identify copy number alterations in single cells.

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Detection Copy Number Alterations Single Cell Sequencing Genomic Heterogeneity Sensitivity Specificity Single Cell Resolution Cytologic Approaches Single-cell Sequencing Genomic Changes Megabase-scale Copy Number Alterations Micro-aspirator PVC Tubing Syringe Filter Plastic Serological Pipette
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