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JoVE Journal Genetics
Påvisning af Copy Number Ændringer Brug Single Cell Sequencing
 
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Påvisning af Copy Number Ændringer Brug Single Cell Sequencing

Article DOI: 10.3791/55143-v • 09:45 min • February 17th, 2017
February 17th, 2017 •
1,2,3, 4, 5
1Koch Institute for Integrative Cancer Research, Department of Biology, Massachusetts Institute of Technology, 2Howard Hughes Medical Institute, 3Division of Health Sciences and Technology, Harvard Medical School, 4The Barbara K. Ostrom (1978) Bioinformatics and Computing Facility in the Swanson Biotechnology Center, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 5BioMicro Center, Department of Biology, Massachusetts Institute of Technology

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Enkelt celle sekventering er en stadig mere populær og tilgængelig redskab til at løse genomiske forandringer i høj opløsning. Vi giver en protokol, der anvender enkelt celle sekventering for at identificere kopital ændringer i enkeltceller.

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Genetik kopiere nummer ombygning aneuploidi genomisk heterogenitet enkelt celle hele genomet forstærkning hele genomet sekventering
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